Diagnostic Test Directory

Virology Laboratory

Hepatitis B virus (HBV) surface antibody - serum or plasma specimen

  • Synonyms: HBsAb, Hepatitis B Surface Antibody
  • LIS Mnemonic: HBVSAB


    PREFERRED: Serum is the preferred specimen. Collect blood in a 4 ml Gold Serum Separator Tube-Clot Activator & Gel. REMARK: Plasma is an acceptable alternative specimen. Blood can be collected in

    Volume Required

    4 ml of blood

    Minimum Required

    2 ml of blood


    A total of 2-4 ml of blood (for every 2-3 Hepatitis and HIV tests ordered) should be collected. For the CHOP enterprise, submit blood specimens as soon as possible at room temperature to Central Laboratory Services. For outside clients, it is recommended that serum or plasma specimens be removed from the clot, red blood cells, or separator gel as soon as possible after collection and before shipping specimens. When shipping serum or plasma specimens, package and label specimens in complian


    Serum or plasma specimens should be stored for no longer than 3 days at room temperature or 7 days at 2-8C following specimen collection and separation from clot, red blood cells, or separator gel. If

    Unacceptable conditions

    Do not use serum or plasma specimens that are heat-inactivated, pooled, grossly hemolyzed, or contain obvious microbial contamination. Performance has not been established for the use of cadeveric specimens or the use of boody fluids other than human serum or plasma.

    Specimen Handling

    Unprocessed blood specimens should routinely be refrigerated at 4C upon arrival in the laboratory. O

Days Performed

Monday thru Friday






Chemiluminescent microparticle immunoassay

Disease Information


The presence of IgG antibody to Hepatitis B virus surface antigen (anti-HBs) indicates past infection or seroconversion following immunization. Anti-HBs generally appears during early convalescence and usually after HBsAg is no longer detectable; low levels of anti-HBs can occasionally be observed in the presence of HBsAg. This antibody persists for life and is a marker of recovery and immunity.


The presence of HBV surface antibody is indicative of recovery from and immunity to infection with HBV or vaccination against HBV. Results >12 mIU/ml

Reference Values

Negative (e.g., <8 mIU/ml) or nonreactive for Hepatitis B Virus (HBV) surface antibody

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