Diagnostic Test Directory

Virology Laboratory

BK Virus PCR (Qualitative) - urine specimen

  • LIS Mnemonic: BKVPCR

    Collect

    Random Urine Container

    Volume Required

    5-10 ml of clean voided urine

    Minimum Required

    5ml of clean voided urine

    Transport

    Keep specimen at 4C

    Processing

    For infants, a volume of 1 to 2 ml of urine is acceptable. If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.

Days Performed

Daily

Reported

Same day

Reflex Testing

Specimens that are positive for BK virus DNA by the qualitative real-time PCR will recieve a quantitative BK virus PCR to determine the copies/ml of BKV DNA within the clinical material.

CPT

87798 (qualitative BK virus PCR); 87799 (quantitative BK virus PCR)

Methodology

Amplification and detection of BK virus DNA using real-time TaqMan PCR and nucleic acid primer/probe pairs specific for conserved regions of the BK virus genome. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as BK virus DNA detected.

Reference Values

Negative or no BK virus DNA detected

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