Diagnostic Test Directory

Virology Laboratory

Herpes simplex virus (HSV) PCR - stool specimen

  • LIS Mnemonic: HSVPCR

    Collect

    Stool in a sterile, leak-proof container

    Volume Required

    2 to 4 ml of liquid stool; 2 to 4 grams of formed stool

    Minimum Required

    2 ml of liquid stool; 2 grams of formed stool

    Transport

    Keep specimen at 4C

    Processing

    The specimen of choice is stool. Rectal swabs are inferior to stool specimens for the detection of viruses and are discouraged. If it is necessary to collect a rectal swab, a sufficient quantity of fecal material (at least a pea-sized amount) should be obtained. If fecal material is not clearly visible on the swab, the specimen is most likely inadequate. Rectal swabs should be immediately placed in viral transport medium.

    Unacceptable conditions

    Swab specimens not received in viral transport medium or received in bacteriological transport medium are discouraged. DO NOT USE CALCIUM ALGINATE OR WOODEN SHAFT SWABS FOR COLLECTION OF SPECIMENS; ONLY USE DACRON OR RAYON TIPPED SWABS ON PLASTIC OR METAL SHAFTS.

Days Performed

Daily

Reported

Same day

Reflex Testing

N/A

CPT

87529

Methodology

Amplification and detection of HSV DNA polymerase gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as herpes simplex virus DNA detected.

Reference Values

Negative or no herpes simplex virus DNA detected

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