Random Urine Container
5-10 ml of clean voided urine
5 ml of clean voided urine
Keep specimen at 4C
For infants, a volume of 1 to 2 ml of urine is acceptable. If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.
When monitoring the urine of immunocompromised patients for BK virus over time, a quantitative PCR assay will be automatically performed on all patients whose urine has been previously positive for BK virus by qualitative and/or quantitative PCR testing.
Amplification and detection of BK virus DNA using real-time TaqMan PCR and nucleic acid primer/probe pairs specific for conserved regions of the BK virus genome. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
BK virus can cause ureteral ulcerations, stenosis, and hemorrhagic cystitis in renal transplant recipients. Persistent active replication of BKV in renal transplant recipients also has been associated with progressive loss of the graft due to BKV-associated nephropathy. BKV-associated hemorrhagic cystitis is a major complication of bone marrow transplants. Continuous monitoring of urine and plasma by real-time PCR is desirable to predict disease and follow recovery over time.
If positive, the quantity of BK virus DNA is reported in both copies/ml and log10 values. Dynamic range of the assay is 302 copies/ml to 600 million
Negative or quantity of BK virus DNA is less than the lower limit of detection
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