Diagnostic Test Directory

Virology Laboratory

Parvovirus B-19 PCR - amniotic fluid specimen

  • Synonyms: Parvovorus PCR, Parvovirus B19 PCR, Parvovirus Real-Time PCR, B19 PCR
  • LIS Mnemonic: PARVOPCR

    Collect

    amniotic fluid

    Volume Required

    1 ml

    Minimum Required

    0.5 ml

    Transport

    Keep specimen at 4C

    Processing

    Amniotic fluid sampling should be done after 21-23 weeks gestation for best sensitivity. Other acceptable specimens include 4-7 ml blood in Gold SST tube for serum or tube with EDTA for plasma, tissue in viral transport medium, bone marrow in EDTA, and CSF.

Days Performed

Daily

Reported

Same day

Reflex Testing

N/A

CPT

87798

Methodology

Amplification and detection of conserved region of parvovirus B19 DNA structural protein genes VP1 and VP2 using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Disease Information

Utility:

Erythema infectiosum (Fifth disease) is the most common manifestation of parvovirus B19 infection. The disease is characterized by a distinctive slapped-cheek" rash that gives the child a flushed look. The rash may also involve the limbs and trunk but can fluctuate in intensity for weeks to months with changes in temperature and exposure to sunlight. Arthralgia and arthritis is common among adults infected with parvovirus B19, particularly women. Polyarthropathy of knees, fingers, and other joints is common. In transient aplastic crisis, the patient has severe anemia characterized by lethargy, pallor, and weakness. Condition occurs as a complication in individuals with chronic hemolytic anemias. Normally lasts 7 to 10 days with complete recovery; however, can be life threatening and blood transfusions may be required to prevent death. Chronic infection resulting in severe anemia has been observed in AIDS patients. Infection during pregnancy can cause fetal hydrops, intrauterine growth retardation, congestive heart failure, and death.

Interpretation

If positive, results are reported as parvovirus B19 DNA detected.

Reference Values

Negative or no parvovirus B19 DNA detected

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