Diagnostic Test Directory

Virology Laboratory

Epstein-Barr Virus (EBV) PCR (Qualitative) - respiratory specimen

  • LIS Mnemonic: EBVPCR


    Bronchoalveolar lavage (BAL)

    Volume Required

    1 ml

    Minimum Required

    0.5 ml


    Keep specimen at 4C


    Bronchoalveolar lavage specimens, bronchial washings, nasal washings, and tracheal aspirates may be submitted. Collection of COMBINED THROAT AND NASOPHARYNGEAL SWABS is recommended for patients in which lavages, aspirates or washings cannot be readily obtained. Immediately transport specimens to the Clinical Virology Laboratory. If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.

    Unacceptable conditions

    Swab specimens not received in viral transport medium or received in bacteriological transport medium are discouraged. DO NOT USE CALCIUM ALGINATE OR WOODEN SHAFT SWABS FOR COLLECTION OF SPECIMENS; ONLY USE DACRON OR RAYON TIPPED SWABS ON PLASTIC OR METAL SHAFTS.

Days Performed



Same day

Reflex Testing





Amplification and detection of EBV DNA of the nonglycosylated membrane protein BNRF1 p143 gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.


If positive, results are reported as Epstein-Barr virus DNA detected.

Reference Values

Negative or no Epstein-Barr virus DNA detected

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