Conjunctival swab in viral transport medium
Keep specimen at 4C
CONJUNCTIVAL SWABS should be taken by stroking the lower conjunctival sac of the eye 5-6 times with a rayon or Dacron tipped, plastic or metal shafted swab to obtain epithelial cells. To facilitate binding of the cellular material to the swab, the swab can be premoistened with sterile saline. Be certain to express the excess liquid from the swab before attempting to collect the specimen. After specimen collection, immediately place swab into tube of viral transport medium and send to the Clinical Virology Laboratory. If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.
Swab specimens not received in viral transport medium or received in bacteriological transport medium are discouraged. DO NOT USE CALCIUM ALGINATE OR WOODEN SHAFT SWABS FOR COLLECTION OF SPECIMENS; ONLY USE DACRON OR RAYON TIPPED SWABS ON PLASTIC OR METAL SHAFTS.
Indirect immunofluorescence assay using primary monoclonal antibodies specific for measles antigens and secondary fluorescein-labeled monoclonal antibodies.
If positive, results are reported as measles (rubeola) virus antigen detected.
Negative or no measles (rubeola) virus antigen detected
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