Diagnostic Test Directory

Virology Laboratory

Parechovirus PCR - stool specimen

  • LIS Mnemonic: PAREPCR


    Stool in a sterile, leak-proof container

    Volume Required

    2 to 4 ml of liquid stool; 2 to 4 grams of formed stool

    Minimum Required

    2 ml of liquid stool; 2 grams of formed stool


    Keep specimen at 4C




    The specimen of choice is stool. Rectal swabs are inferior to stool specimens for the detection of viruses and are discouraged. If it is necessary to collect a rectal swab, a sufficient quantity of fecal material (at least a pea-sized amount) should be obtained. If fecal material is not clearly visible on the swab, the specimen is most likely inadequate. Rectal swabs should be immediately placed in viral transport medium. Specimens should be transported to the laboratory as quickly as possible after collection. When immediate transport is not possible, refrigerate the specimens or keep them on wet ice. For delays of more than 24-48 hours (e.g., when shipping specimens from an outside facility), specimens should be processed as needed and rapidly frozen to -70C and then transported to the laboratory on dry ice.

    Unacceptable conditions

    Specimens for molecular testing should not be stored at room temperature or frozen at -20C. This is critical to ensure the stability and amplification of nucleic acids, particularly for the detection of RNA viruses since RNA is unstable and easily degraded by RNAses from the surrounding environment.

Days Performed



Same day

Reflex Testing





Amplification and detection of parechovirus RNA from the highly conserved 5'-untranslated region of the genome using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.


If positive, results are reported as parechovirus RNA detected.

Reference Values

Negative or no parechovirus RNA detected

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