Tissue specimen in viral transport medium
punch biopsy size
Keep specimen at 4C
Specimens should be transported to the laboratory as quickly as possible after collection. When immediate transport is not possible, refrigerate the specimens or keep them on wet ice. For delays of more than 24-48 hours (e.g., when shipping specimens from an outside facility), specimens should be processed as needed and rapidly frozen to -70C and then transported to the laboratory on dry ice.
Tissue not in viral transport medium. Also, specimens for molecular testing should not be stored at room temperature or frozen at -20C. This is critical to ensure the stability and amplification of nucleic acids, particularly for the detection of RNA viruses since RNA is unstable and easily degraded by RNAses from the surrounding environment.
Amplification and detection of parechovirus RNA from the highly conserved 5'-untranslated region of the genome using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
If positive, results are reported as parechovirus RNA detected.
Negative or no parechovirus RNA detected
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