Diagnostic Test Directory

Virology Laboratory

Parechovirus PCR - tissue specimen - e.g., colon, liver, myocardium

  • LIS Mnemonic: PAREPCR

    Collect

    Tissue specimen in viral transport medium

    Volume Required

    N/A

    Minimum Required

    punch biopsy size

    Transport

    Keep specimen at 4C

    Stability

    N/A

    Processing

    Specimens should be transported to the laboratory as quickly as possible after collection. When immediate transport is not possible, refrigerate the specimens or keep them on wet ice. For delays of more than 24-48 hours (e.g., when shipping specimens from an outside facility), specimens should be processed as needed and rapidly frozen to -70C and then transported to the laboratory on dry ice.

    Unacceptable conditions

    Tissue not in viral transport medium. Also, specimens for molecular testing should not be stored at room temperature or frozen at -20C. This is critical to ensure the stability and amplification of nucleic acids, particularly for the detection of RNA viruses since RNA is unstable and easily degraded by RNAses from the surrounding environment.

Days Performed

Daily

Reported

Same day

Reflex Testing

N/A

CPT

87798

Methodology

Amplification and detection of parechovirus RNA from the highly conserved 5'-untranslated region of the genome using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as parechovirus RNA detected.

Reference Values

Negative or no parechovirus RNA detected

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