Diagnostic Test Directory

Microbiology Laboratory

Stool culture

  • Synonyms: Gastrointestinal Culture
  • LIS Mnemonic: CGI

    Collect

    Stool, colonic aspirate, colonic wash

    Rectal swab on patients < 2 yrs of age if stool is not possible

    Refer to Stability and Transport for appropriate submission

    Optimal Submission: specimen in Ent

    Volume Required

    Liquid specimen: approximately 5 ml in ETM or 1 tbsp in sterile container

    Minimum Required

    Same as volume required

    Transport

    Unpreserved stool/colonic wash/aspirate in sterile container: immediately at room temperature

    Stool in Enteric Transport Media (ETM) or rectal swab: ASAP at room temperature

    DO NOT REFRIGERATE OR FREEZE

    Stability

    Unpreserved Stool: must be received in Lab within 30 minutes of collection.

    Specimen in Enteric Transport Media: must be received in the Lab within 72 hours of collection.

    Processing

    Stool pathogens routinely cultured for: Salmonella, Shigella, Campylobacter, Yersinia, Aeromonas,and E.coli 0157:H7. Any other organism needs a special request. This request also includes testing for E.coli/Shiga Toxin testing

    Unacceptable conditions

    Unpreserved stools received >30 minutes after collection; diapers containing stool; refrigerated or frozen specimens;

    swabs on patients > 2 yrs of age;

    this specimen is not appropriate for anaerobic culture.

Days Performed

Daily

Reported

Updates Daily Until Final

Reflex Testing

N/A

CPT

87045

Methodology

Culture