Diagnostic Test Directory

Michael J. Palmieri Metabolic Disease Laboratory

Non Esterified Free Fatty Acid (NEFFA)

  • LIS Mnemonic: NEFFA

    Collect

    Gold (SST - Clot activator & gel)

    Volume Required

    2.0 mL

    Minimum Required

    1.0 mL

    Transport

    Room temperature

    Stability

    N/A

    Processing

    Place specimens in rack for 30 minutes prior to centrifugation to allow for complete clotting of sample. Specimens collected in a SST should be centrifuged at 3,400 RPM's for 10 minutes. If there is gel separating the serum from the red cells, it is not necessary to transfer the serum to another tube. Follow directions in the centrifuge manual to set the centrifuge appropriately.If there is not gel separating the serum from the red cells, transfer the serum using a disposable pipette to another tube (pour off tube) being careful not to disturb the buffy coat. Rubber band the original tube (parent tube) with the labeled pour off tube prior to sending the specimen to CLS.

    Unacceptable conditions

    plasma specimen is unacceptable

Days Performed

Bi-weekly

Reported

2-3 weeks

Reflex Testing

N/A

CPT

82725

Methodology

enzymatic spectrophotometric assay

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