Diagnostic Test Directory

Immunology Laboratory

Complement C4

  • LIS Mnemonic: C4

    Collect

    Gold (SST - Clot activator & gel)

    Volume Required

    4.0 ml

    Minimum Required

    2.0 ml

    Transport

    On ice

    Stability

    Must be delivered on ice to CLS/Immunology Lab within 24 hours.

    Processing

    Allow specimen to clot on ice for 1 hour prior to centrifugation. All immunology specimens collected in an SST should be centrifuged at 3,400 RPM's for 10 minutes. If there is gel separating the serum from the red cells, it is not necessary to transfer the serum to another tube. Follow directions in the centrifuge manual to set the centrifuge appropriately. If there is not gel separating the serum from the red cells, transfer the serum using a disposable pipette to another labeled tube (pour off tube) being careful not to disturb the buffy coat. Specimen must be kept on ice after centrifugation and for delivery to the lab. Rubber band the original tube (parent tube) with the labeled pour off tube prior to sending the specimen to CLS/Immunology Lab. NOTE: Results may be adversely affected if specimen is not collected and processed on ice.

Days Performed

Monday, Wednesday & Thursday

Reported

2 days

Reflex Testing

N/A

CPT

86160

Methodology

Nephelometry

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