Diagnostic Test Directory

Clinical Chemistry Laboratory

Methotrexate

  • LIS Mnemonic: MTX

    Collect

    Gold (SST - Clot activator & gel) Satellite locations must send Gold top tube

    Volume Required

    1 mL blood

    Minimum Required

    0.5 mL blood

    Transport

    Protect from light

    Stability

    4 hrs room temp, 24 hrs refrigerated

    Processing

    Place specimens in rack for 30 minutes prior to centrifugation to allow for complete clotting of sample. All chemistry specimens collected in a SST or PST should be centrifuged at 3,400 RPM's for 10 minutes. If there is gel separating the serum or plasma from the red cells, it is not necessary to transfer the serum or plasma to another tube. Follow directions in the centrifuge manual to set the centrifuge appropriately.If there is not gel separating the serum or plasma from the red cells, transfer the serum or plasma using a disposable pipette to another tube (pour off tube) being careful not to disturb the buffy coat. Rubber band the original tube (parent tube) with the labeled pour off tube prior to sending the specimen to CLS.

Days Performed

daily

Reported

Same day

Reflex Testing

N/A

CPT

80299

Methodology

TDX

Search Lab Test Directory

Search by disease or test name

View a full list of all lab tests

Contact Us

Ask for more information about our laboratory services.

215-590-1823