Diagnostic Test Directory

Virology Laboratory

HIV-1/2 rapid antibody test - plasma specimen

  • Synonyms: Rapid HIV-1/2 Antibody Test, HIV Rapid Test, Rapid HIV Test
  • LIS Mnemonic: RAHIV


    PREFERRED: Plasma is the preferred specimen. Blood can be collected in plastic tubes containing dipotassium EDTA with or without gel separator (either a lavender top tube or a pearl top plasma prepar

    Volume Required

    4 ml of blood

    Minimum Required

    2 ml of blood


    For the CHOP enterprise, submit blood specimens as soon as possible at room temperature to Central Laboratory Services. For outside clients, it is recommended that plasma specimens be removed from the red blood cells or separator gel as soon as possible after collection and before shipping specimens. When shipping plasma specimens, package and label specimens in compliance with applicable state, federal, and international regulations covering the transport of clinical specimens and infectious


    Plasma specimens should be stored for no longer than 3 days at room temperature or 7 days at 2-8C following specimen collection and separation from red blood cells or separator gel. If a storage perio

    Unacceptable conditions

    Do not use serum or plasma specimens that are heat-inactivated, pooled, grossly hemolyzed, or contain obvious microbial contamination.

    Specimen Handling

    Unprocessed blood specimens should routinely be refrigerated at 4C upon arrival in the laboratory. O

Days Performed

Daily (Mon thru Su) on Day Shift; Mon thru Fri on Evening Shift


Same shift

Reflex Testing

All positive rapid HIV-1/2 test results require additional testing for confirmation. A conventional laboratory-based screening immunoassay and Western blot as well as an HIV-1 RNA qualitative test will be ordered and performed by the laboratory to confirm the initially reactive rapid test results.




Rapid Immunochromatographic Assay

Disease Information


The Rapid HIV-1/2 Antibody Test is ued by the laboratory to test source patients that have been involved in accidental exposures of healthcare workers to blood and body fluids and to test mothers receiving care at the Center for Fetal Diagnosis and Treatment and delivering their babies in the Special Delivery Unit. Employees involved in an accidental exposure to blood or body fluids from a source patient must contact Occupational Health and get prior authorization to order the test. The test can then be ordered as part of the Occupational Health Order Set in EPIC.


A positive result using the Rapid HIV-1/2 Antibody Test is presumptive and suggests the presence of HIV-1 and/or HIV-2 antibodies in the specimen. Ad

Reference Values

Negative for HIV-1 and HIV-2 antibodies by rapid test

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