Diagnostic Test Directory

Virology Laboratory

Herpes simplex virus (HSV) PCR - dermal lesion specimen

  • LIS Mnemonic: HSVPCR

    Collect

    Dermal lesion swab in viral transport medium

    Volume Required

    N/A

    Minimum Required

    N/A

    Transport

    Keep specimen at 4C

    Processing

    Specimen Collection from Dermal Lesions: Cells and fluid from fresh vesicles are superior to specimens prepared from other lesion types (e.g vesicles >> pustules >> ulcers >> crusts) for direct antigen detection, PCR and viral culture. Uncap the vesicle with a sterile needle or scalpel blade and use a sterile rayon or Dacron tipped, plastic shafted swab to vigorously swab the base of the lesion to obtain infected epithelial cells. To facilitate binding of the cellular material to the swab, the swab can be premoistened with sterile saline. Be certain to express the excess liquid from the swab before attempting to collect the specimen. For ulcers, use a swab to remove pus without disrupting the lesion base, and then use a fresh sterile swab to vigorously swab the base of the lesion to obtain cells. Crusted lesions should have the crust removed and discarded before collection of cells from the lesion base. NOTE: The yield of HSV from a crusted lesion is quite low for rapid antigen and culture-based tests and moderate for PCR, so the utility of collecting specimens from this type of lesion should be strongly considered. The swab should be immediately placed in viral transport medium and sent to the Clinical Virology Laboratory.

    Unacceptable conditions

    Swab specimens not received in viral transport medium or received in bacteriological transport medium are discouraged. DO NOT USE CALCIUM ALGINATE OR WOODEN SHAFT SWABS FOR COLLECTION OF SPECIMENS; ONLY USE DACRON OR RAYON TIPPED SWABS ON PLASTIC OR METAL SHAFTS.

Days Performed

Daily

Reported

Same day

Reflex Testing

N/A

CPT

87529

Methodology

Amplification and detection of HSV DNA polymerase gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as herpes simplex virus DNA detected.

Reference Values

Negative or no herpes simplex virus DNA detected

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