Diagnostic Test Directory

Virology Laboratory

BK Virus PCR (Qualitative) - blood plasma specimen

  • LIS Mnemonic: BKVPCR

    Collect

    Lavendar (EDTA)

    Volume Required

    4-7 ml; for infants a volume of 2-4 ml is acceptable

    Minimum Required

    4 ml; for infants a volume of 2 ml is acceptable

    Transport

    Keep specimen at 4C

    Unacceptable conditions

    Clotted specimens

Days Performed

Daily

Reported

Same day

Reflex Testing

Specimens that are positive for BK virus DNA by the qualitative real-time PCR will receive a quantitative BK virus PCR to determine the copies/ml of BKV DNA within the clinical material.

CPT

87798 (qualitative BK virus PCR); 87799 (quantitative BK virus PCR)

Methodology

Amplification and detection of BK virus DNA using real-time TaqMan PCR and nucleic acid primer/probe pairs specific for conserved regions of the BK virus genome. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as BK virus DNA detected.

Reference Values

Negative or no BK virus DNA detected

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