Diagnostic Test Directory

Virology Laboratory

Adenovirus PCR - eye (conjunctival) specimen

  • LIS Mnemonic: ADENOPCR

    Collect

    Conjunctival swab in viral transport medium

    Volume Required

    N/A

    Minimum Required

    N/A

    Transport

    Place swab in Viral Transport Medium and keep specimen at 4C immediately after collection and during storage and transport. The laboratory provides transport medium to all CHOP patient areas and outside clients upon request.

    Processing

    CONJUNCTIVAL SWABS should be taken by stroking the lower conjunctival sac of the eye 5-6 times with a rayon or Dacron tipped, plastic or metal shafted swab to obtain epithelial cells. To facilitate binding of the cellular material to the swab, the swab can be premoistened with sterile saline. Be certain to express the excess liquid from the swab before attempting to collect the specimen. After specimen collection, immediately place swab into tube of viral transport medium and send to the Clinical Virology Laboratory. If an extended delay in transport of specimens is anticipated, rapidly freeze the specimens to at least -60°C and transport to the laboratory on dry ice. Please consult the laboratory if necessary.

    Unacceptable conditions

    Swab specimens not received in viral transport medium or received in bacteriological transport medium are discouraged. DO NOT USE CALCIUM ALGINATE OR WOODEN SHAFT SWABS FOR COLLECTION OF SPECIMENS; ONLY USE DACRON OR RAYON TIPPED SWABS ON PLASTIC OR METAL SHAFTS.

Days Performed

Daily

Reported

Same day

Reflex Testing

N/A

CPT

87798

Methodology

Amplification and detection of adenovirus DNA using real-time TaqMan PCR and nucleic acid primer/probe pairs specific for conserved regions of the adenovirus genome. Detects all 51 known serotypes of adenoviruses; does not differentiate by type. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as adenovirus DNA detected.

Reference Values

Negative or no adenovirus DNA detected

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