Diagnostic Test Directory

Virology Laboratory

Human Herpes Virus-6 (HHV-6) PCR (Qualitative) - blood specimen

  • LIS Mnemonic: HHV6PCR

    Collect

    Lavendar (EDTA)

    Volume Required

    4-7 ml whole blood

    Minimum Required

    4 ml whole blood

    Transport

    Transport blood in a timely fashion (preferably within 8 hours of collection) at room temperature to the Clinical Virology Laboratory.

    Stability

    N/A

    Processing

    Whole blood in an EDTA-anticoagulated tube is the preferred specimen source. Thoroughly mix the blood by gently inverting the collection tube 6-12 times before sending to the laboratory. Other acceptable specimens include body fluids, tissue submitted in viral transport medium, bone marrow in EDTA, and CSF.

    Unacceptable conditions

    Clotted specimens

Days Performed

Daily

Reported

Same day

Reflex Testing

If qualitative HHV-6 DNA real-time PCR is positive from blood, a quantitative assay will be performed to measure the amount of HHV-6 DNA present.

CPT

87532

Methodology

Amplification and detection of HHV-6 DNA U65-U66 gene region using TaqMan real-time PCR technology. The test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as human herpesvirus-6 DNA detected.

Reference Values

Negative or no human herpesvirus-6 DNA detected

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