4-7 ml whole blood
4 ml whole blood
Transport blood in a timely fashion (preferably within 8 hours of collection) at room temperature to the Clinical Virology Laboratory.
Whole blood in an EDTA-anticoagulated tube is the preferred specimen source. Thoroughly mix the blood by gently inverting the collection tube 6-12 times before sending to the laboratory. Other acceptable specimens include body fluids, tissue submitted in viral transport medium, bone marrow in EDTA, and CSF.
If qualitative HHV-6 DNA real-time PCR is positive from blood, a quantitative assay will be performed to measure the amount of HHV-6 DNA present.
Amplification and detection of HHV-6 DNA U65-U66 gene region using TaqMan real-time PCR technology. The test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
If positive, results are reported as human herpesvirus-6 DNA detected.
Negative or no human herpesvirus-6 DNA detected
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