Diagnostic Test Directory

Virology Laboratory

Epstein-Barr Virus (EBV) PCR (Qualitative) - blood specimen

  • LIS Mnemonic: EBVPCR

    Collect

    Lavendar (EDTA)

    Volume Required

    4-7 ml whole blood

    Minimum Required

    4 ml whole blood

    Transport

    Transport blood in a timely fashion (preferably within 8 hours of collection) at room temperature to the Clinical Virology Laboratory.

    Stability

    N/A

    Processing

    Whole blood in an EDTA-anticoagulated tube is the preferred specimen source. Thoroughly mix the blood by gently inverting the collection tube 6-12 times before sending to the laboratory. Other acceptable specimens include body fluids, tissue submitted in viral transport medium, and CSF.

    Unacceptable conditions

    Clotted specimens

Days Performed

Daily

Reported

Same day

Reflex Testing

If qualitative EBV PCR assay is positive, a quantitative EBV PCR assay will be performed to measure the amount of EBV DNA present.

CPT

87798

Methodology

Amplification and detection of EBV DNA of the nonglycosylated membrane protein BNRF1 p143 gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Interpretation

If positive, results are reported as Epstein-Barr virus DNA detected.

Reference Values

Negative or no Epstein-Barr virus DNA detected

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