4-7 ml whole blood
4 ml whole blood
Transport blood in a timely fashion (preferably within 8 hours of collection) at room temperature to the Clinical Virology Laboratory.
Whole blood in an EDTA-anticoagulated tube is the preferred specimen source. Thoroughly mix the blood by gently inverting the collection tube 6-12 times before sending to the laboratory. Other acceptable specimens include body fluids, tissue submitted in viral transport medium, and CSF.
If qualitative EBV PCR assay is positive, a quantitative EBV PCR assay will be performed to measure the amount of EBV DNA present.
Amplification and detection of EBV DNA of the nonglycosylated membrane protein BNRF1 p143 gene using TaqMan real-time PCR technology. This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.
If positive, results are reported as Epstein-Barr virus DNA detected.
Negative or no Epstein-Barr virus DNA detected
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