Blood collected in a blood culture bottle (Maximum 5 mLs per bottle).
<1.5 weight in kg = 1 mL total blood volume needed = 1 bottle
1.5-3.9 weight in kg = 1 mL total blood volume needed = 1 bottle
4-7 weight in kg = 3 mL total blood needed = 2 bottles
8-13 weight in kg = 6 mL total blood needed = 3 bottles
14-18 weight in kg = 12 mL total blood needed = 3 bottles
19-25 weight in kg = 16 mL total blood needed = 4 bottles
26-39 weight in kg = 20 mL total blood needed = 5 bottles
>40 weight in kg = 32 mL total blood needed = 8 bottles
1% of patient total blood volume (see above chart for calculations)
Depends on weight of patient (see above chart for calculations)
Send to the lab immediately at room temperature
Bottles stable up to 2 hours after collection
Bottles should not be refrigerated. Stoppers should not be removed from bottle. All needles should be removed from bottle before sending to the lab.
Updated daily until final (final as negative in 10 days)
Divide among multiple bottles if total draw is > 5 mLs.
Refer to information located under "Clinical Laboratories" for the Microbiology section: Forms and Documents. Document: "Guidelines on Blood Culture for Subacute Bacterial Endocarditis".