Diagnostic Test Directory

Immunogenetics Laboratory

High Resolution HLA-A,B,C,DRB1,DQB1 Typing

  • Synonyms: HLA Hi Res-A,B,C,DRB1,DQB1 (EPIC)
  • LIS Mnemonic: HLAHIGHPROF

    Collect

    Lavender (EDTA), Cord Blood (1 ml), or 4 buccal brushes

    Volume Required

    4ml

    Minimum Required

    1ml (4ml if low WBC) or 4 buccal brushes

    Transport

    Room temperature

    Stability

    72 hrs

    Processing

    Buccal Brush Collection Instructions: Collect tissue by rolling the Buccal Brush firmly on the inside of the cheek, approximately 20 times on each side, making certain to move the brush over the entire cheek. 4 brushes should be collected per individual. Allow brushes to air dry for 10-15 minutes at room temperature. Return the dry brushes to their plastic sleeves and send to the laboratory.

    Unacceptable conditions

    Samples older than 72 hours. Mislabeled samples: Samples must be labeled with at least two different identifiers, date & time of collection, and collector's signature.

Days Performed

Monday- Friday (7:30AM- 5:00PM)

Reported

10 days

Reflex Testing

N/A

CPT

81378, 81382

Methodology

Sequence-Based Typing (SBT)

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215-590-5648